Mitosis as an anti-cancer target (2) - Movies

Human cells going through mitosis:

 

        In order to visualize subcellular structures under the microscope we make use of fluorescent "tags". This allows us to make movies of these structures. In these cells microtubules appear green (GFP-tubulin) and chromosomes red (Cherry-HistoneH2B). First, we will see some examples of normally dividing cells:

 

 The first movie shows how the MTOCs (microtubule organizing centres) separate to opposite sides of the cell to organise the bipolar spindle.

 

 

The second movie shows a human cell going through division:

 

 

 

 

Human cells treated with Taxol (Paxiclatel)

 

Now compare with these drug-treated cells:

Taxol stabilises microtubules. This taxol-treated cell does not form a bipolar spindle and "arrests" in mitosis:

 

 

This movie shows a taxol-treated cell arresting and dying in mitosis: